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For normal control muscle, this 1:20 dilution will give a protein loading of approx 200 μg in 30 μL (7). Abbreviations: AHAanterior hypothalamic area; BDBdiagonal band of Broca; BLbasolateral nucleus of the amygdala; BSTbed nucleus of the stria terminalis; cgcingulum; Ciinferior colliculus; Cxcortex; CPucaudate putamen; DeNdorsoendopiriform nucleus; DMHdorsomedial nucleus of the hypo- thalamus; DTN dorsal tegmental nucleus; EMeminentia mediana; Ententorhinal cortex; GDgyrus dentatus; GPglobus pallidus; Hihippocampus; LAlateral nu- cleus of the amygdala; LClocus coeruleus; MCLHnucleus magnocellularis of the lateral hypothalamus; NRLlateral nucleus reticularis; OPTolivary pretectal nucleus; PVNparaventricular nucleus of the hypo- thalamus; VP ventral pallidum; VTA ventral tegmental area; Xnucleus of the nervus vagus.
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